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Abstract

Ascospore segregants ("slime"-like) of the triple mutant fz(fuzzy);sg(spontaneous germination) os-1(osmotic) ("slime"; Emerson 1963. Genetica 34:162-182) of Neurospora crassa germinate as a plasmodium which, after some time, results in a morphologically abnormal mycelium. If the mycelium of a "slime"-like isolate is cultured under high osmotic pressure (Nelson et al. 1975. Neurospora Newsl. 22:15-16), it releases cells lacking walls which proliferate as spheroplasts.

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