It is difficult to obtain progeny when N. crassa and N. tetrasperma are intercrossed directly. Metzenberg and Ahlgren (1969 Neurospora Newsl. 15:9-10; 1973 Can. J. Genet. Cytol. 15:571-576) developed a transfer kit of interspecific hybrids which they used for bridging-crosses that enabled them to move the mating type genes from N. tetrasperma into N. crassa. I have recently been concerned with introgressing mutant genes and translocations from N. crassa into N. tetrasperma, and have found their strain C4,T4 a (FGSC 1778) extremely useful for the initial cross, and more fertile than other members of the kit.
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Perkins, D. D.
"Transfer of genes and translocations from Neurospora crassa to N. tetrasperma,"
Fungal Genetics Reports:
Vol. 38, Article 14.