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Abstract

Gene cloning in Neurospora crassa is often achieved by mutant complementation. However, the cloning strategy sometimes requires the isolation of a specific genomic region (by chromosome walking) before transformation of N. crassa. This is the case, for example, if the gene to be isolated has a non-selectable phenotype. Here we specifically describe the construction of the cosmid vector, pAC3, which is designed for direct transformation of N. crassa, its utilization for the construction of a genomic library, and chromosome walking in the region of un-10 on linkage group VII.

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