Humicola grisea is a thermophilic, cellulolytic fungus, with significant biotechnological potential for protein secretion. For convenient growth on solid medium, 70% reduction in colony diameter was achieved by the addition of 20 mM sodium citrate to modified Aspergillus complete medium (Pontecorvo et al. 1953, Adv. Genet. 5:141-238).
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MACEDO, J. M.,
"Techniques for colonial growth and protoplast production in Humicola grisea var. thermoidea,"
Fungal Genetics Reports:
Vol. 38, Article 9.