Transformation of Magnaporthe grisea to phosphinothricin resistance using the bar gene from Streptomyces hygroscopicus

Authors

Hei Leung, Washington State University
Pat Loomis, Washington State University
Martin L. Pall, Washington State University

Abstract

Three transformation systems have been reported for the rice blast fungus Magnaporthe grisea (Parsons et al. 1987 Proc. Natl. Acad. Sci. USA 84:4161-4165; Daboussi et al. 1989 Curr. Genet. 15:453-456; Leung et al. 1990 Curr. Genet. 17:409-411). Among these three selection systems, only hygromycin B resistance provides a dominant selection that can be used for any wild type strain. A second dominant selection marker is needed to transform strains that are already hygromycin B resistant.

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Recommended Citation

Leung, H., P. Loomis, and M.L. Pall (1995) "Transformation of Magnaporthe grisea to phosphinothricin resistance using the bar gene from Streptomyces hygroscopicus," Fungal Genetics Reports: Vol. 42, Article 11. https://doi.org/10.4148/1941-4765.1341