Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In Saccharomyces cerevisiae, PCR assay can be performed with yeast colonies (Huxley et al. TIG 1990 6:236). Here we describe a PCR protocol which uses M. grisea conidia directly for PCR analysis without extraction of DNA.
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"Assessment of Magnaporthe grisea mating type by spore PCR,"
Fungal Genetics Reports:
Vol. 42, Article 28.