Evidence of evolutionary change in the rust, Puccinia grindeliae, populations in New Mexico and Arizona, is revealed by use of PCR amplified ITS regions of rDNA. Polymorphic ITS5 - ITS2 fragments were found both within a single community and among geographically and temporally separated collections. These results provide evidence for genetic heterogeneity, possibly resulting from recent evolutionary change in reproductive strategy from clonal to sexual.
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"PCR amplification of ITS rDNA from rust teliospores collected on southwestern rangeland from 1907 to 1995,"
Fungal Genetics Reports:
Vol. 43, Article 16.