In order to circumvent the problem in assigning the mating types of some N. discreta strains by fluffy testers, a PCR based method was developed. Between the two reference N. discreta strains (FGSC 3228 A and FGSC 4378 a), we observed the false perithecia to be more abundant in the "a" mating type. When we analyzed the octads from a cross of these two, the "false perithecia" appear to be associated with a particular morphology and mating type.
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Adhvaryu, K. K.,
"A simple method to determine mating type in Neurospora discreta,"
Fungal Genetics Reports:
Vol. 48, Article 2.