A Ligation-PCR Approach for Generating Gene Replacement Constructs in Magnaporthe grisea

Authors

Xinhua Zhao, Purdue University
Chaoyang Xue, Purdue University
Yangseon Kim, Purdue University
Jin-Rong Xu, Purdue University

Abstract

The conventional approach for generating gene replacement constructs involves several sequence-specific cloning steps and is time-consuming. A ligation-PCR approach was developed to efficiently generate gene replacement constructs. Two vectors useful for this ligation-PCR approach and another vector suitable for improving the efficiency of knockout mutant screens were constructed.

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Recommended Citation

Zhao, X., C. Xue, Y. Kim, and J. Xu (2004) "A Ligation-PCR Approach for Generating Gene Replacement Constructs in Magnaporthe grisea," Fungal Genetics Reports: Vol. 51, Article 7. https://doi.org/10.4148/1941-4765.1137