Boil-mediated lysis of Neurospora conidia (Boil-prep) is an extremely rapid, convenient and useful technique to obtain sufficient genomic DNA template for PCR amplification. We routinely use this technique for screening molecular markers, sequencing, and preliminary confirmation of transformants.
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Henderson, S. T.,
"Reliable PCR amplification from Neurospora crassa genomic DNA obtained from conidia,"
Fungal Genetics Reports:
Vol. 52, Article 7.