RNA extraction, probe preparation, and competitive hybridization for transcriptional profiling using Neurospora crassa long-oligomer DNA microarrays

Authors

Travis A. Clark, Yale University
Julie M. Guilmette, Yale University
Daniel Renstrom, University of Connecticut
Jeffrey P. Townsend, Yale University

Abstract

We developed protocols optimized for the performance of experiments assaying genomic gene expression using Neurospora crassa long-oligomer microarrays. We present methods for sample growth and harvesting, total RNA extraction, poly(A)⁺ mRNA selection, preparation of NH₃-Allyl Cy3/Cy5 labeled probes, and microarray hybridization. The quality of the data obtained with these protocols is demonstrated by the comparative transcriptional profiling of basal and apical zones of vegetative growth of N. crassa.

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Recommended Citation

Clark, T. A., J.M. Guilmette, D. Renstrom, and J.P. Townsend (2008) "RNA extraction, probe preparation, and competitive hybridization for transcriptional profiling using Neurospora crassa long-oligomer DNA microarrays," Fungal Genetics Reports: Vol. 55, Article 6. https://doi.org/10.4148/1941-4765.1086