Tetrad analysis may be accomplished either with ordered asci squeezed from perithecia, or with asci shot as an unordered group at a target slab of agar (Perkins 1966, Neurospora Newsl. 9:11).
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Metzenberg, R. L.
"An alternate way of collecting, storing, and dissecting Neurospora asci.,"
Fungal Genetics Reports:
Vol. 35, Article 15.