A new acetate-requiring mutant strain of Neurospora crassa (ace-9), has been isolated from the double mutant strain cot-1;inl a, by inositol-less death (Lester and Gross 1959. Science 129:572) in Vogel's medium supplemented with 0.3% sodium acetate and 2% sucrose. The mutant grows well on complex medium and on Vogel's medium supplemented with casamino acids, acetate, or acetate plus ethanol. Like ace-2, ace-3 and ace-4 (Okumura and Kuwana 1979. Japan J. Genet. 54:235-244), it shows very weak activity of pyruvate dehydrogenase compelx, but has normal activities of pyruvate carboxylase, pyruvate kinase and glucose-6-phosphate dehydrogenase.

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