Until now, maintenance of Podospora anserina strains was tedious, requiring yearly sexual crosses, spore isolation and germination, verification of the genotype and transfer of mycelium to stock tubes maintained at 4 C. We have circumvented these difficulties by storage of ascospores at -80 C.
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"Long term storage of Podospora anserina,"
Fungal Genetics Reports:
Vol. 38, Article 2.