A qa2+-pGEM vector for Neurospora transformations

Authors

M. A. Nelson, University of Wisconsin
R. L. Metzenberg, University of Wisconsin

Abstract

The qa-2+ gene has been widely used as a selectable marker in Neurospora transformations (Akins and Lambowitz 1985. Mol. Cell. Biol. 5:2272-2278). A plasmid (pMSN1) has been constructed to facilitate the cloning of selected genes into a qa-2+ vector. The system takes advantage of the blue/white screening possible when cloned fragments are inserted into the E. coli lacZ (beta-galactosidase) gene, and also facilitates subsequent sequencing of the cloned genes.

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Recommended Citation

Nelson, M. A., and R.L. Metzenberg (1992) "A qa2+-pGEM vector for Neurospora transformations," Fungal Genetics Reports: Vol. 39, Article 11. https://doi.org/10.4148/1941-4765.1437