It is well known that multiple auxotrophic markers impede fruiting in Coprinus cinereus. Restriction fragment length polymorphisms have been used to advantage (Cassidy et al. 1984 Curr. Genet. 8:607-613; Zolan et al. 1992 Nucl. Acids Res. 20:3993-3999) but the required Southern analysis is both time consuming and laborious.
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"Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction,"
Fungal Genetics Reports:
Vol. 40, Article 11.