Expression vectors for Neurospora crassa and expression of a bovine preprochymosin cDNA

Authors

Eileen T. Nakano, University of Hawaii
Raymond D. Fox, University of Hawaii
David E. Clements, University of Hawaii
Ken Koo, University of Hawaii
W. Dorsey Stuart, University of Hawaii
John M. Ivy, University of Hawaii

Abstract

The filamentous fungi, owing to their ability to secrete high levels of proteins, are attractive organisms for the expression and secretion of heterologous proteins of commercial and medical value. We report the construction of three expression vectors for the production of heterologous proteins in Neurospora crassa and demonstrate their utility by expression of a bovine preprochymosin cDNA and secretion of processed, enzymatically active bovine chymosin.

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Recommended Citation

Nakano, E. T., R.D. Fox, D.E. Clements, K. Koo, W.D. Stuart, and J.M. Ivy (1993) "Expression vectors for Neurospora crassa and expression of a bovine preprochymosin cDNA," Fungal Genetics Reports: Vol. 40, Article 19. https://doi.org/10.4148/1941-4765.1410