Assessment of Magnaporthe grisea mating type by spore PCR

Authors

Jin-Rong Xu, Purdue University
John E. Hamer, Purdue University

Abstract

Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In Saccharomyces cerevisiae, PCR assay can be performed with yeast colonies (Huxley et al. TIG 1990 6:236). Here we describe a PCR protocol which uses M. grisea conidia directly for PCR analysis without extraction of DNA.

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Recommended Citation

Xu, J., and J.E. Hamer (1995) "Assessment of Magnaporthe grisea mating type by spore PCR," Fungal Genetics Reports: Vol. 42, Article 28. https://doi.org/10.4148/1941-4765.1358