A protocol for electroporating Neurospora strains is described. This protocol uses spheroplasts for transformation rather than germinated conidia with enzyme-weakened walls. High efficiency transformation is obtained with this system even with batches of Novozyme234 which worked poorly in the PEG based protocol.
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Kothe, G. O.,
"Protocol for the Electroporation of Neurospora Spheroplasts,"
Fungal Genetics Reports:
Vol. 43, Article 13.