We have been using lacZ as a reporter gene in N. crassa. The standard -galactosidase assay can be labor intensive and time consuming when large numbers of strains are assayed simultaneously. We sought a technique to simplify the pipetting steps involved in assay preparation and in optical density reading. High reproducibility and rapid processing was obtained by adapting the standard test tube method to a microassay performed in a 96-well microplate.
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"A reliable microplate assay for determination of B-galactosidase activity in Neurospora crassa strains bearing lacZ fusions,"
Fungal Genetics Reports:
Vol. 43, Article 15.