A mutant gene is most simply transferred from killer to nonkiller by crossing a marked killer strain x Sk sensitive acr-2R, plating ascospores on medium supplemented with acriflavine, and recovering mutant progeny among the acriflavine-resistant colonies, which are nonkillers that presumably originated from rare heterozygous or heterokaryotic ascospores.
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Perkins, D. D.
"Methods for extracting markers from Spore killer strains of Neurospora crassa,"
Fungal Genetics Reports:
Vol. 43, Article 19.