After transformation of Aspergillus nidulans with plasmid DNA the transformants are usually incubated at 37C until transformants appear. We have found that pre-incubation of the transformation plates at room temperature for 24h leads to increased transformation frequencies.
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Cockram, J. A.,
"A simple modification of the A. nidulans transformation protocol increases the transformation frequency,"
Fungal Genetics Reports:
Vol. 47, Article 24.