The conventional approach for generating gene replacement constructs involves several sequence-specific cloning steps and is time-consuming. A ligation-PCR approach was developed to efficiently generate gene replacement constructs. Two vectors useful for this ligation-PCR approach and another vector suitable for improving the efficiency of knockout mutant screens were constructed.
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"A Ligation-PCR Approach for Generating Gene Replacement Constructs in Magnaporthe grisea,"
Fungal Genetics Reports:
Vol. 51, Article 7.