An improved plasmid for transformation of Neurospora crassa using the pan-2 gene as a selectable marker

Authors

Wilson Low, National University of Singapore
Gregory Jedd, National University of Singapore

Abstract

The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as a selective marker. We mapped the pan-2 fragment to the right arm of LG VI, contig 4, and found that it carries a pan-2 linked gene, atg-12 (NCU10049.3). We report the removal of atg-12 to produce an improved plasmid, pOKE104.

Creative Commons License

This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License.

Recommended Citation

Low, W., and G. Jedd (2008) "An improved plasmid for transformation of Neurospora crassa using the pan-2 gene as a selectable marker," Fungal Genetics Reports: Vol. 55, Article 11. https://doi.org/10.4148/1941-4765.1091