The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as a selective marker. We mapped the pan-2 fragment to the right arm of LG VI, contig 4, and found that it carries a pan-2 linked gene, atg-12 (NCU10049.3). We report the removal of atg-12 to produce an improved plasmid, pOKE104.
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"An improved plasmid for transformation of Neurospora crassa using the pan-2 gene as a selectable marker,"
Fungal Genetics Reports:
Vol. 55, Article 11.