Student Major/Year in School
Medical Biochemistry, First year
Faculty Mentor Information
Phillip E Klebba, Biochemistry and Molecular Physics, College of Arts and Sciences
Abstract
The focus of my research is to create a clone of a Heme transporter from Caulobacter crescentus and transformed into E. coli OKN359 and fluorescently label it so that it can detect Heme in the environment. This sensor will be combined with others in a fluorescence assay to analyze pathogenic bacteria and identify drugs that are the most effective in inhibiting their iron transport. To do so, I used Gibson cloning and made a hybrid gene, pITS27, that contains a small initial portion of an E.coli gene fepA followed by the full Caulobacter crescentus gene hutA. The initial portion of the E.coli gene was necessary to ensure proper insertion of the Caulobacter crescentus HutA protein in the outer membrane.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
Recommended Citation
Tokgozoglu, Gloriana (2019). "Heme Expression from Caulobacter Crescentus in E. coli," Kansas State University Undergraduate Research Conference. https://newprairiepress.org/ksuugradresearch/2019/posters/61
Heme Expression from Caulobacter Crescentus in E. coli
The focus of my research is to create a clone of a Heme transporter from Caulobacter crescentus and transformed into E. coli OKN359 and fluorescently label it so that it can detect Heme in the environment. This sensor will be combined with others in a fluorescence assay to analyze pathogenic bacteria and identify drugs that are the most effective in inhibiting their iron transport. To do so, I used Gibson cloning and made a hybrid gene, pITS27, that contains a small initial portion of an E.coli gene fepA followed by the full Caulobacter crescentus gene hutA. The initial portion of the E.coli gene was necessary to ensure proper insertion of the Caulobacter crescentus HutA protein in the outer membrane.
