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Abstract

A rapid method for the detection of transforming sequences in a fungal strain would be advantageous when trying to determine if unselected sequences are present. Colony hybridization protocols for filamentous fungi have been developed (Stohl and Lambowitz, 1983. Anal. Biochem. 134:82-85; Paietta and Marzluf, 1984. Neurospora Newsletter 31:40) and a modified method thereof was described (McClung and Dunlap, 1988. Fungal Genetics Newsletter 35:26-27). In this report a simple method for the isolation of fungal DNA from a single transformed colony suitable for dot blot analysis is described. If the original transformant colony is not too small it is sufficient to extract the DNA of one half of that colony. That means that no subculturing is necessary and the results can be available within 24 h starting from the transformant colony.

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