Role of the cell wall on the expression of osmotic-sensitive (os-1) and temperature-sensitive (cot-1) phenotypes of N. crassa. A comparative study on mycelial and wall-less phenotypes of the "slime" variant

Authors

M. A. NOVENTA, Universidade de São Paulo
J. A. JORGE, Universidade de São Paulo
H. F. TERENZI, Universidade de São Paulo

Abstract

Ascospore segregants ("slime"-like) of the triple mutant fz(fuzzy);sg(spontaneous germination) os-1(osmotic) ("slime"; Emerson 1963. Genetica 34:162-182) of Neurospora crassa germinate as a plasmodium which, after some time, results in a morphologically abnormal mycelium. If the mycelium of a "slime"-like isolate is cultured under high osmotic pressure (Nelson et al. 1975. Neurospora Newsl. 22:15-16), it releases cells lacking walls which proliferate as spheroplasts.

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Recommended Citation

NOVENTA, M. A., J.A. JORGE, and H.F. TERENZI (1991) "Role of the cell wall on the expression of osmotic-sensitive (os-1) and temperature-sensitive (cot-1) phenotypes of N. crassa. A comparative study on mycelial and wall-less phenotypes of the "slime" variant," Fungal Genetics Reports: Vol. 38, Article 10. https://doi.org/10.4148/1941-4765.1456