A simple, rapid procedure for the isolation of DNA for PCR from Gibberella fujikuroi (Fusarium section Liseola)

Authors

N. M. DuTEAU, Kansas State University
J. F. LESLIE, Kansas State University

Abstract

The polymerase chain reaction (PCR) is a method for amplifying specific segments of DNA defined by the small primers used to start the reaction. Using arbitrarily chosen 10-base primers, one can generate "random amplified polymorphic DNA" (RAPD) markers (Williams et al. 1991 Nucl. Acids Res. 18:6531-6535). These DNA fragments, separated by electrophoresis in an agarose gel, can be used as markers for studying genetic variation within and among fungal populations.

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Recommended Citation

DuTEAU, N. M., and J.F. LESLIE (1991) "A simple, rapid procedure for the isolation of DNA for PCR from Gibberella fujikuroi (Fusarium section Liseola)," Fungal Genetics Reports: Vol. 38, Article 5. https://doi.org/10.4148/1941-4765.1451