Filtration-enrichment methods for selecting auxotrophs and other mutants in Ascobolus immersus and similar filamentous fungi.

Authors

B. C. LAMB, Imperial College of Science
S. HELMI, University of Alexandria

Abstract

Filtration-enrichment is a very successful method for obtaining auxotrophs, but traditional methods cannot be applied directly to many fungi lacking suitable asexual spores, mainly because of a heavy carry-over of parental nutrients into sexual spores. A procedure has been devised to apply filtration-enrichment to such fungi by using hyphal mutagenesis, crossing, germination of sexual spores, fragmentation of germination hyphae into small propagules, and then two cycles of filtration-enrichment in liquid minimal medium (LMM).

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Recommended Citation

LAMB, B. C., and S. HELMI (1991) "Filtration-enrichment methods for selecting auxotrophs and other mutants in Ascobolus immersus and similar filamentous fungi.," Fungal Genetics Reports: Vol. 38, Article 7. https://doi.org/10.4148/1941-4765.1453