Techniques for colonial growth and protoplast production in Humicola grisea var. thermoidea

Authors

J. M. B. MACEDO, Leeds University
J. L. AZEVEDO, Instituto de Genetica

Abstract

Humicola grisea is a thermophilic, cellulolytic fungus, with significant biotechnological potential for protein secretion. For convenient growth on solid medium, 70% reduction in colony diameter was achieved by the addition of 20 mM sodium citrate to modified Aspergillus complete medium (Pontecorvo et al. 1953, Adv. Genet. 5:141-238).

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Recommended Citation

MACEDO, J. M., and J.L. AZEVEDO (1991) "Techniques for colonial growth and protoplast production in Humicola grisea var. thermoidea," Fungal Genetics Reports: Vol. 38, Article 9. https://doi.org/10.4148/1941-4765.1455