Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction

Authors

T. Freedman, University of North Carolina
P. J. Pukkila, University of North Carolina

Abstract

It is well known that multiple auxotrophic markers impede fruiting in Coprinus cinereus. Restriction fragment length polymorphisms have been used to advantage (Cassidy et al. 1984 Curr. Genet. 8:607-613; Zolan et al. 1992 Nucl. Acids Res. 20:3993-3999) but the required Southern analysis is both time consuming and laborious.

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Recommended Citation

Freedman, T., and P.J. Pukkila (1993) "Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction," Fungal Genetics Reports: Vol. 40, Article 11. https://doi.org/10.4148/1941-4765.1402