A reliable microplate assay for determination of B-galactosidase activity in Neurospora crassa strains bearing lacZ fusions

Authors

Kwangwon Lee, Texas A&M University
Daniel J. Ebbole, Texas A&M University

Abstract

We have been using lacZ as a reporter gene in N. crassa. The standard -galactosidase assay can be labor intensive and time consuming when large numbers of strains are assayed simultaneously. We sought a technique to simplify the pipetting steps involved in assay preparation and in optical density reading. High reproducibility and rapid processing was obtained by adapting the standard test tube method to a microassay performed in a 96-well microplate.

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Recommended Citation

Lee, K., and D.J. Ebbole (1996) "A reliable microplate assay for determination of B-galactosidase activity in Neurospora crassa strains bearing lacZ fusions," Fungal Genetics Reports: Vol. 43, Article 15. https://doi.org/10.4148/1941-4765.1311