PCR amplification of ITS rDNA from rust teliospores collected on southwestern rangeland from 1907 to 1995

Authors

Craig Liddell, New Mexico State University
Kathy Onsurez Waugh, New Mexico State University

Abstract

Evidence of evolutionary change in the rust, Puccinia grindeliae, populations in New Mexico and Arizona, is revealed by use of PCR amplified ITS regions of rDNA. Polymorphic ITS5 - ITS2 fragments were found both within a single community and among geographically and temporally separated collections. These results provide evidence for genetic heterogeneity, possibly resulting from recent evolutionary change in reproductive strategy from clonal to sexual.

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Recommended Citation

Liddell, C., and K.O. Waugh (1996) "PCR amplification of ITS rDNA from rust teliospores collected on southwestern rangeland from 1907 to 1995," Fungal Genetics Reports: Vol. 43, Article 16. https://doi.org/10.4148/1941-4765.1312