Identification of a cosmid clone containing the Neurospora crassa lys-5 and un-4 genes, isolation of a partial lys-5 cDNA and associated chromosome walking.

Authors

Thomas J. Schmidhauser, The University of Southwestern Louisiana
Dan Chen, The University of Southwestern Louisiana
Yihong Wan, University of Colorado

Abstract

The un-4 gene of Neurospora crassa was cloned to determine the limits of a chromosome walk on linkage group VI (LGVI) and to allow analysis of un loci on LGVI. Subsequent analysis identified the lys-5 locus on the same cosmid clone as un-4. We have isolated and sequenced a partial lys-5 cDNA clone and initiated a chromosome walk from the lys-5, un-4 cosmid clone.

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Recommended Citation

Schmidhauser, T. J., D. Chen, and Y. Wan (1999) "Identification of a cosmid clone containing the Neurospora crassa lys-5 and un-4 genes, isolation of a partial lys-5 cDNA and associated chromosome walking.," Fungal Genetics Reports: Vol. 46, Article 10. https://doi.org/10.4148/1941-4765.1238