The use of beta-D-glucanase as a substitute for Novozyme 234 in immunofluorescence and protoplasting

Authors

M Katherine Jung, The Ohio State University
Yulia Ovechkina, The Ohio State University
Natalie Prigozhina, The Ohio State University
C Elizabeth Oakley, The Ohio State University
Berl R. Oakley, The Ohio State University

Abstract

Novozym 234 has been used for many years to prepare protoplasts of Aspergillus nidulans and other fungi for transformation. It has also been very useful in immunofluorescence studies for partially digesting walls of fixed hyphae or germlings to allow antibodies to penetrate into the cytoplasm. In recent years, the availability of Novozym 234 has become problematic, and we have searched for combinations of available enzymes that are suitable for protoplasting and immunofluorescence studies in A. nidulans.

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Recommended Citation

Jung, M. K., Y. Ovechkina, N. Prigozhina, C.E. Oakley, and B.R. Oakley (2000) "The use of beta-D-glucanase as a substitute for Novozyme 234 in immunofluorescence and protoplasting," Fungal Genetics Reports: Vol. 47, Article 8. https://doi.org/10.4148/1941-4765.1204