Multiple cotransformations in Coprinus cinereus

Authors

Ursula Kües, Institut für Mikrobiologie
Michaela J. Klaus, Institut für Mikrobiologie
Eline Polak, Institut für Mikrobiologie
Markus Aebi

Abstract

Plasmids usually integrate ectopically into the genome of the homobasidiomycete Coprinus cinereus in transformations. Often, integration occurs at multiple sites indicating that more than one plasmid copy was incorporated. This feature prompted us to study transformation with mixtures of several different plasmids in several genetic backgrounds. We found multiple cotransformation to be efficient even with four different plasmids. Simultaneous uptake of a second plasmid was between 5-55%, of two additional plasmids between 3-18% and 3% for three additional plasmids. These high frequencies make possible the analysis of interactions between different heterologous genes introduced into the same nucleus.

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Recommended Citation

Kües, U., M.J. Klaus, E. Polak, and M. Aebi (2001) "Multiple cotransformations in Coprinus cinereus," Fungal Genetics Reports: Vol. 48, Article 11. https://doi.org/10.4148/1941-4765.1176