Expression and visualization of Green Fluorescent Protein (GFP) in Neurospora crassa

Authors

Michael Freitag, University of Oregon
Lynda M. Ciuffetti, Oregon State University
Eric U. Selker, University of Oregon

Abstract

We report the first successful imaging of GFP expression in Neurospora crassa. GFP was expressed under the control of the heterologous ToxA promoter from Pyrenophora tritici-repentis in transformants carrying multiple or single copies of the GFP construct. GFP was also detected in ascospores but not during earlier stages of the sexual cycle.

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Recommended Citation

Freitag, M., L.M. Ciuffetti, and E.U. Selker (2001) "Expression and visualization of Green Fluorescent Protein (GFP) in Neurospora crassa," Fungal Genetics Reports: Vol. 48, Article 7. https://doi.org/10.4148/1941-4765.1172