A GATEWAY™ Destination Vector For High-Throughput Construction of Neurospora crassa histidine-3 Gene Replacement Plasmids

Authors

Jeremy R. Haag, Washington University
Dong W. Lee, Texas A&M University
Rodolfo Aramayo, Texas A&M University

Abstract

We report the construction of a Destination Vector, called pJHAM007, for the targeted integration of DNA sequences at the histidine-3 (his-3) locus of Neurospora crassa. pJHAM007 has all the necessary features required to perform a simple, rapid and efficient GATEWAY™ recombinational cloning with an Entry Clone to yield a his-3-gene replacement Destination Vector.

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Recommended Citation

Haag, J. R., D.W. Lee, and R. Aramayo (2003) "A GATEWAY™ Destination Vector For High-Throughput Construction of Neurospora crassa histidine-3 Gene Replacement Plasmids," Fungal Genetics Reports: Vol. 50, Article 3. https://doi.org/10.4148/1941-4765.1149