Isolation of genomic DNA from acetone-dried Aspergillus mycelia

Authors

N. S. Punekar, Indian Institute of Technology
S. V. Suresh Kumar, Indian Institute of Technology
T. N. Jayashri, Indian Institute of Technology
R. Anuradha, Indian Institute of Technology

Abstract

A simple acetone-drying protocol was adopted to replace the lyophilization step while isolating genomic DNA from Aspergillus mycelia. This DNA is suitable for PCR, restriction enzyme digestion and Southern blot analysis with digoxigenin-labeled DNA probes. Acetone drying/ preservation can be a useful method in the molecular analysis of fungal DNA samples.

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Recommended Citation

Punekar, N. S., S.V. Suresh Kumar, T.N. Jayashri, and R. Anuradha (2003) "Isolation of genomic DNA from acetone-dried Aspergillus mycelia," Fungal Genetics Reports: Vol. 50, Article 7. https://doi.org/10.4148/1941-4765.1153