A precise size-estimate for the small RNA products arising from Neurospora crassa Dicer activity

Authors

Paul ReFalo, Oregon Health & Science University
Matthew S. Sachs, Oregon Health & Science University

Abstract

Neurospora crassa cell-free extracts prepared from strains containing one or both functional Dicer genes, but not from a strain lacking functional Dicer genes, converts radiolabeled double-strand RNA (dsRNA) in an energy-dependent manner into short RNAs with an estimated size of ~25-nt (Catalanotto et al. 2004). A smaller nucleolytic digestion product was also produced in an energy-dependent manner from either dsRNA or single-stranded RNA. Here we obtained more precise sizes for these products by electrophoresis of samples on a long (40-cm) denaturing DNA sequencing gel (20% polyacrylamide/7M urea).

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Recommended Citation

ReFalo, P., and M.S. Sachs (2004) "A precise size-estimate for the small RNA products arising from Neurospora crassa Dicer activity," Fungal Genetics Reports: Vol. 51, Article 9. https://doi.org/10.4148/1941-4765.1139