Disruption of a Yeast ADE6 Gene Homolog in Ustilago maydis

Authors

M. L. Heidenreich, University of Wisconsin
A. D. Budde, United States Department of Agriculture
An Zhiqiang, University of Wisconsin
S. A. Leong, University of Wisconsin

Abstract

A putative homolog of the Sacharromyces cereviseae ADE6 and Escherichia coli purL genes is identified near a multigenic complex, which contains two genes, sid1 and sid2, involved in a siderophore biosynthetic pathway inUstilago maydis. The putative ADE6 homolog was mutated by targeted gene disruption. The resulting mutant strains demonstrated a requirement for exogenous adenine, indicating that the U. maydis ade6 homolog is required for purine biosynthesis.

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Recommended Citation

Heidenreich, M. L., A.D. Budde, A. Zhiqiang, and S.A. Leong (2008) "Disruption of a Yeast ADE6 Gene Homolog in Ustilago maydis," Fungal Genetics Reports: Vol. 55, Article 10. https://doi.org/10.4148/1941-4765.1090