Strategies for the molecular genetic manipulation and visualization of the human fungal pathogen Penicillium marneffei

Authors

Kylie J. Boyce, The University of Melbourne
Hayley E. Bugeja, The University of Melbourne
Harshini Weerasinghe, The University of Melbourne
Michael J. Payne, The University of Melbourne
Lena Schreider, The University of Melbourne

Abstract

P. marneffei has been established as an experimentally amenable system to study morphogenesis and pathogenicity. This paper describes the development of a number of tools, including numerous selectable markers, to expand the ease with which it can be genetically manipulated. Combined with strains engineered for homologous recombination of exogenous DNA, these tools facilitate efficient molecular genetic studies.

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Recommended Citation

Boyce, K. J., H.E. Bugeja, H. Weerasinghe, M.J. Payne, and L. Schreider (2012) "Strategies for the molecular genetic manipulation and visualization of the human fungal pathogen Penicillium marneffei," Fungal Genetics Reports: Vol. 59, Article 1. https://doi.org/10.4148/1941-4765.1009