Natural isolates of Neurospora which cannot be vegetatively propagated continuously have been identified among collections from distant geographical locations (X. Yang and A.J.F. Griffiths 1993 Mol. Gen. Genet. 237:177-186). We have found that senescence is common in strains of N. intermedia growing on burned sugar cane in fields in Maddur, approximately 80 km southwest of Bangalore. In a survey of 150 strains, 29 strains have died to date in 57 serial subcultures made at intervals of 5-10 days on slants of Vogel's N medium + 1.5% sucrose. Senescence was first observed after a minimum of 9 subcultures. Nearly 30 more strains have lost their vigor or have become aconidial.

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