Long term storage of R. solani was improved by using the freezing and thawing conditions that are generally used for animal/human cell lines. Key factors to success were the use of controlled freezing and the use of complete mycelial colonies.
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"Long Term Storage of Isolates of Rhizoctonia Solani, a Mycelial Fungus,"
Fungal Genetics Reports:
Vol. 43, Article 12.