Evidence of evolutionary change in the rust, Puccinia grindeliae, populations in New Mexico and Arizona, is revealed by use of PCR amplified ITS regions of rDNA. Polymorphic ITS5 - ITS2 fragments were found both within a single community and among geographically and temporally separated collections. These results provide evidence for genetic heterogeneity, possibly resulting from recent evolutionary change in reproductive strategy from clonal to sexual.

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