Methods for extracting markers from Spore killer strains of Neurospora crassa

Authors

D. D. Perkins, Stanford University

Abstract

A mutant gene is most simply transferred from killer to nonkiller by crossing a marked killer strain x Sk sensitive acr-2R, plating ascospores on medium supplemented with acriflavine, and recovering mutant progeny among the acriflavine-resistant colonies, which are nonkillers that presumably originated from rare heterozygous or heterokaryotic ascospores.

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Recommended Citation

Perkins, D. D. (1996) "Methods for extracting markers from Spore killer strains of Neurospora crassa," Fungal Genetics Reports: Vol. 43, Article 19. https://doi.org/10.4148/1941-4765.1315