A rapid method for isolation of stable niaD and crnA mutants of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae

Authors

S. S. Sandhu, Osmania University
G. Venkateswerlu, Osmania University

Abstract

Generally niaD mutants of fungi are selected by spontaneous mutations on appropriate minimal medium supplemented with various concentrations of KClO3 and a nitrogen source (Daboussi et al. 1989 Curr. Genet. 15:453-456; Johnstone et al. 1990 Gene 90:181-192; Malardier et al.1989 Gene 78:147-156; Unkles et al.1989 Gene 78:157-166). But in case of entomopathogenic fungi it has been observed that niaDmutants isolated simply by spontaneous mutation on chlorate were not stable, (Table-1). Therefore a method has been developed to isolate stable niaD mutants of these fungi by treating protoplasts with ethane methane sulfonate (EMS).

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Recommended Citation

Sandhu, S. S., and G. Venkateswerlu (1997) "A rapid method for isolation of stable niaD and crnA mutants of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae," Fungal Genetics Reports: Vol. 44, Article 18. https://doi.org/10.4148/1941-4765.1286