NPP > JOURNALS > FGR > Vol. 49 (2002)
A quick method to isolate pure DNA from asexual spores of Coprinus cinereus for screening approaches
Abstract
In this study, we present a glass bead-phenol method to isolate genomic DNA from oidia of the basidiomycete Coprinus cinereus. The DNA can be used in Southern blot analysis with digoxigenin-labelled DNA probes without the background problems encountered with DNA isolated from fungal mycelium. Furthermore, DNA isolated from oidia can be applied in PCR. This is especially useful when searching for specific DNA sequences or recombination events in a mixture of different strains.
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Recommended Citation
Walser, P. J.,
U. Kües,
and
M. Aebi
(2002)
"A quick method to isolate pure DNA from asexual spores of Coprinus cinereus for screening approaches,"
Fungal Genetics Reports:
Vol. 49, Article 6.
https://doi.org/10.4148/1941-4765.1188
