An effect of homology length on gene disruption in Neurospora crassa

Authors

Y. Nakanishi, Saitama University
C. Ishii, Saitama University
H. Inoue, Saitama University

Abstract

For targeted gene disruption in wild-type Neurospora crassa, 1000-bp of homologous sequences on either side of the cassette used for disruption is sufficient to give more than 10 % homologous recombination. We report here that varying the length of homology on each side seems to have different effects on the homologous recombination frequency.

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Recommended Citation

Nakanishi, Y., C. Ishii, and H. Inoue (2005) "An effect of homology length on gene disruption in Neurospora crassa," Fungal Genetics Reports: Vol. 52, Article 1. https://doi.org/10.4148/1941-4765.1120