Reliable PCR amplification from Neurospora crassa genomic DNA obtained from conidia

Authors

Steven T. Henderson, Flinders University
Graham A. Eariss, Flinders University
David E. A. Catcheside, Flinders University

Abstract

Boil-mediated lysis of Neurospora conidia (Boil-prep) is an extremely rapid, convenient and useful technique to obtain sufficient genomic DNA template for PCR amplification. We routinely use this technique for screening molecular markers, sequencing, and preliminary confirmation of transformants.

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Recommended Citation

Henderson, S. T., G.A. Eariss, and D.E. Catcheside (2005) "Reliable PCR amplification from Neurospora crassa genomic DNA obtained from conidia," Fungal Genetics Reports: Vol. 52, Article 7. https://doi.org/10.4148/1941-4765.1126